Fig. 3. Aggresomal degradation pathway. HEK-293 cell lines stably expressing (A) A1038V, or (B) G1961E were treated for 16 h with increasing doses of the aggresome inhibitor tubacin. Data are normalized to the 0 µM control. *P<0.05. n=3-4. Ezrin was used as the loading control. The A1038 V and G1961E mutants are significantly degraded in the aggresome.